Abstract the filamentous fungus, aspergillus niger mtcc 282, was used to produce citric acid in submerged fermentation with colocassia antiquorum (10% w/v) as the substrate the maximum yield of citric acid (465 mg ml-1) was obtained with inoculum age (7 days), inoculum level (2% v/v), temperature (30°c) , ph (40). Summary the influence of various carbon sources and their concentration on the production of citrate by aspergillus niger has been investigated the sugars maltose, sucrose, glucose, mannose and fructose (in the given order) were carbon sources giving high yields of citric acid optimal yields were observed at sugar. A solid state fermentation was developed for citric acid production from pineapple waste by aspergillus niger ks-7 the medium was supplemented with different concentration of glucose, sucrose, ammonium nitrate and ammonium phosphate it was found that pineapple waste with 15% (w/v) sucrose and ammonium nitrate. Citric acid production by aspergillus niger matic legiša and majda gradišnik-grapulin national institute of chemistry, slo-61115 ljubljana, slovenia received 19 october 1994/accepted 22 april 1995 on the basis of the present knowledge of aspergillus niger metabolism during citric acid fermentation. Summary the production of citric acid from fermentation medium (mass per volume ratio 001 % of undersized semolina) by aspergillus niger was studied by shake culture method the effects of initial ph (45, 55 and 65), methanol ( volume fraction 10, 20 and 30 %) and ammonium nitrate (mass per volume ratio 001. The present investigation deals with the kinetics of submerged citric acid fermentation by aspergillus niger using blackstrap molasses as the basal fermentation media a laboratory scale stirred fermentor of 15-l capacity having working volume of 9-l was used for cultivation process and nutritional analysis among the 10. The factors which govern citric acid production by aspergillus niger in sub- merged culture, particularly the influence of the metal constituents of the medium, were investigated in detail by shu & johnson (1947, 1948a, b), and their findings were confirmed and extended by tomlinson, campbell & trussell (1950, 1951. Aims: citric acid is a commercially important acid that has many applications in varying sectors of industries it is produced by various substrates through solid state or submerged fermentation the capabilities of potato and rice as substrates for citric acid production using aspergillus niger were tested in this experiment.
Citric acid is a commercially important product used in several industries processes production of citric acid by aspergillus niger from different varieties of banana peel such as yellow banana in surface culture process was studied it was found that the maximum amount of citric acid (87%) was produced from yellow. Citric acid has high economic potential owing to its numerous applications it is mostly produced by microbial fermentation using aspergillus niger in view of surges in demand and growing markets, there is always a need for the discovery and development of better production techniques and solutions to. Efficient production of citric acid in segmented fermentation using aspergillus niger based on recycling of a pellet-dispersion strategy† baoshi wangabc, jian chena, hua lia, fuxin sunc, youran liab and guiyang shiab anational engineering laboratory for cereal fermentation technology, wuxi.
The study was conducted to investigate the potential of parkia biglobosa fruit pulp as substrate for citric acid production by aspergillus niger reducing sugar was estimated by 3,5-dinitrosalicylic acid and citric acid was estimated spectrophotometrically using pyridine-acetic anhydride methods the studies. Objective: the present study made an attempt to produce commercially valuable citric acid by the fungal strain aspergillus niger from molasses and corncob using submerged fermentation, as the best alternative to the sugar substrate methods: three types of production media were prepared including control (sucrose) by. Food additive: functional use(s) - solvent extraction process for citric acid.
Citric acid (ca) is the most important commercial product which is produced by using various sugar substrates in the terrestrial environment the present study made an attempt to produce citric acid by the fungal strain aspergillus niger from red seaweed gelidiella acerosa is the best alternative to sugar. Martin,3 in a very recent study of the citric acid fermentation, discarded all cultures of aspergilli with the assump- tion that their fermentative action was well known and that they did not produce citric acid it has been noted4 that many cultures of aspergillus niger produced citric acid although the literature on the chemical.
Aspergillus niger strains were isolated from various sources like soil, floor, window glass and microbiology lab these strains were screened for the production of citric acid and the strain that produced maximum amount of citric acid was selected and identified based on its cultural characteristics, employing slide culture. Doi: 1013189/ujmr2016040401 optimization of citric acid production using aspergillus niger isolated from the leaf litter soil of sathuragiri hills thiruvengadam shankar, thangavel sivakumar department of microbiology, ayya nadar janaki ammal college (autonomous), india. Citric acid is the most important organic acid produced in tonnage and is extensively used in food and pharmaceutical industries it is produced mainly by submerged fermentation using aspergillus niger or candida sp from different sources of carbohydrates, such as molasses and starch based media however, other.
Production of citric acid by aspergillus niger using cane molasses in a stirred fermentor sikander ali biotechnology research laboratories department of botany government college lahore, pakistan tel: 92 42 9211634 fax: 92 42 7243198 e-mail: [email protected] ikram-ul-haq biotechnology. Although many microorganisms can be employed to produce citric acid, a niger is still the main industrial producer in fact, specific strains that are able to overproduce citric acid in different types of fermentation processes have been developed the theoretical yield is 112 g of anhydrous citric acid per 100. The proposal involves the metabolic modelling of citric acid and itaconic acid production in a niger based on transcript profiling, sub-cellular proteomics and sub-cellular metabolomics cellular imaging data would provide input into the model for cellular localization of enzyme activities and the sub-cellular ph at the side of. Citric acid accumulation is known to be stimulated by high sugar concentration suggesting it may be influenced by osmotic stress a possible role of protein phosphorylation due to osmotic stress has not been investigated in a niger under citric acid production conditions the goal of this study was to investigate.